- Proteomic studies of differentially expressed proteins in roots after inocculation of resistant and susceptible hops with lethal pathotype of Verticillium albo-atrum
We analyzed proteomic profile of hop roots in control and fungus-infected plants in three time points after inoculation. Responses of susceptible and resistant cultivar were then compared. The profile changed between time points significantly also in control plants, therefore the influences of infection and time were separated using appropriate statistic methods. We found out that infection caused significant quantitative changes in more than a fifth of detectable proteome in susceptible cultivar, while there were no changes in resistant cultivar. Mass spectrometry was used to identify 100 protein spots. As expected, increased production of defence proteins was found in susceptible cultivar, whereas specific forms of a lectin potentially involved in pathogen recognition were identified in resistant cultivar.
- MANDELC, Stanislav, TIMPERMAN, Isaak, RADIŠEK, Sebastjan, DEVREESE, Bart, SAMYN, Bart, JAVORNIK, Branka. Comparative proteomic profiling in compatible and incompatible interactions between hop roots and Verticillium albo-atrum. Plant physiol. biochem. (Paris). [Print ed.], 2013, doi: 10.1016/j.plaphy.2013.03.017.
- CREGEEN, Sara, RADIŠEK, Sebastjan, MANDELC, Stanislav, TURK, Boris, ŠTAJNER, Nataša, JAKŠE, Jernej, JAVORNIK, Branka. Different gene expressions of resistant and susceptible hop cultivars in response to infection with a highly aggressive strain of Verticillium albo-atrum. Plant molecular biology reporter, ISSN 0735-9640, 2014, str. v tisku, doi: 10.1007/s11105-014-0767-4.
- ŠTAJNER, Nataša, CREGEEN, Sara, JAVORNIK, Branka. Evaluation of reference genes for RT-qPCR expression studies in hop (Humulus lupulus L.) during infection with vascular pathogen Verticillium albo-atrum. PloS one, ISSN 1932-6203, 2013, vol. 8, issue 7, str. 1-13 (e68228), ilustr. http://dx.doi.org/10.1371/journal.pone.0068228, doi: 10.1371/journal.pone.0068228.